Altered expression of plant lysyl tRNA synthetase promotes tRNA misacylation and translational recoding of lysine.

Xing Rong Wu, Alexander Kenzior, David Willmot, Sarah Scanlon, Zhihong Chen, Andrey Topin, Shao Hua He, Ashley Acevedo, William R Folk

Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA.

The Plant journal : for cell and molecular biology 2007 May

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The Arabidopsis thaliana lysyl tRNA synthetase (AtKRS) structurally and functionally resembles the well-characterized prokaryotic class IIb KRS, including the propensity to aminoacylate tRNA(Lys) with suboptimal identity elements, as well as non-cognate tRNAs. Transient expression of AtKRS in carrot cells promotes aminoacylation of such tRNAs in vivo and translational recoding of lysine at nonsense codons. Stable expression of AtKRS in Zea mays causes translational recoding of lysine into zeins, significantly enriching the lysine content of grain.

Citation

Xing Rong Wu, Alexander Kenzior, David Willmot, Sarah Scanlon, Zhihong Chen, Andrey Topin, Shao Hua He, Ashley Acevedo, William R Folk. Altered expression of plant lysyl tRNA synthetase promotes tRNA misacylation and translational recoding of lysine. The Plant journal : for cell and molecular biology. 2007 May;50(4):627-36