tRNAPhe cleavage by aminoglycosides is triggered off by formation of an abasic site.

Jan Wrzesiński, Wojciech Szczepanik, Jerzy Ciesiołka, Małgorzata Jezowska-Bojczuk

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.

Biochemical and biophysical research communications 2005 May 27

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This communication reports the characteristics of the mechanism of highly specific tRNA(Phe) cleavage, which occurs in the anticodon loop in the presence of aminoglycoside antibiotic-neomycin B. The data prove that the cleavage requires previous depurination of the polynucleotide chain at position 37, which is occupied by a hypermodified guanine base-wybutine. The results suggest that the phenomenon, previously considered as selective with respect to the presence of tRNA hypermodification, may concern far more RNA molecules, namely the ones carrying abasic sites.

Citation

Jan Wrzesiński, Wojciech Szczepanik, Jerzy Ciesiołka, Małgorzata Jezowska-Bojczuk. tRNAPhe cleavage by aminoglycosides is triggered off by formation of an abasic site. Biochemical and biophysical research communications. 2005 May 27;331(1):267-71