Quantification of cysteine S-conjugate of 3-sulfanylhexan-1-ol in must and wine of petite arvine vine by stable isotope dilution analysis.

Jean-Luc Luisier, Hermann Buettner, Sebastian Völker, Thierry Rausis, Urban Frey

Institute of Life Technologies, University of Applied Sciences Western Switzerland Valais, Route de Rawyl 64, CH-1950 Sion 2, Switzerland.

Journal of agricultural and food chemistry 2008 May 14

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Making use of a convenient synthetic approach to prepare the deuterated S-3-(hexan-1-ol)-cysteine by a Michael addition reaction, an analytical method was developed to measure the presence of the cysteine S-conjugate, precursor of 3-sulfanylhexan-1-ol (3-mercaptohexan-1-ol), in must and wine from Petite Arvine vine. The method uses a stable isotope dilution assay with a suitable one-step sample preparation and HPLC-MS detection. The method has limits of detection and quantification of 3 and 10 microg/L, respectively. A correlation between the increase of the precursor concentration and the increase of the degree of rot has been established.

Citation

Jean-Luc Luisier, Hermann Buettner, Sebastian Völker, Thierry Rausis, Urban Frey. Quantification of cysteine S-conjugate of 3-sulfanylhexan-1-ol in must and wine of petite arvine vine by stable isotope dilution analysis. Journal of agricultural and food chemistry. 2008 May 14;56(9):2883-7