Development of balanced medium composition for improved rifamycin B production by isolated Amycolatopsis sp. RSP-3.

To develop optimum fermentation environment for enhanced rifamycin B production by isolated Amycolatopsis sp. RSP-3. The impact of different fermentation parameters on rifamycin B production by isolated Amycolatopsis sp. RSP-3 was investigated using Taguchi methodology. Controlling fermentation factors were selected based on one variable at a time methodology. The isolated strain revealed more than 25% higher production compared to literature reports. Five different nutritional components (soyabean meal, glucose, potassium nitrate, calcium carbonate and barbital) and inoculum concentration showed impact on rifamycin B production at individual and interactive level. At optimized environment, 65% contribution was observed from selected fermentation parameters. Soyabean meal and calcium carbonate were the most significant factors among the selected factors followed by barbital and potassium nitrate. Glucose, however, showed the least significance on rifamycin B production with this strain. A maximum of 5.12 g l(-1) rifamycin B production was achieved with optimized medium containing (g l(-1)) soyabean meal, 27; glucose, 100; potassium nitrate, 4; calcium carbonate, 3 and barbital, 1.2. The present study signifies identification of balanced medium component concentrations for improved rifamycin B production by isolated Amycolatopsis sp. RSP-3. This strain requires organic and inorganic nitrogen sources for effective product yield. Yet at individual level, organic nitrogen source has c. nine-fold higher influence compared to inorganic one.

Citation

Y Mahalaxmi, T Sathish, R S Prakasham. Development of balanced medium composition for improved rifamycin B production by isolated Amycolatopsis sp. RSP-3. Letters in applied microbiology. 2009 Nov;49(5):533-8

Mesh Tags

Substances

PMID: 19793193

search → result