Hydrolysis and speciation of Al bound to pectin and plant cell wall material and its reaction with the dye chrome azurol S.

J Bernhard Wehr, F P C Blamey, J V Hanna, P M Kopittke, G L Kerven, N W Menzies

School of Land, Crop and Food Sciences, The University of Queensland, St. Lucia, Queensland, Australia. b.wehr@uq.edu.au

Journal of agricultural and food chemistry 2010 May 12

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Hydrolysis of aluminum (Al) in solution increases at pH >or= 4 and with an Al concentration. Pectin, an important anionic polysaccharide of plant cell walls, adsorbs Al, but this phenomenon is poorly understood. This study showed that Al(3+) hydrolysis results in binding of Al to pectin in excess of the stoichiometric equivalent, leading to oversaturation of the pectin with Al. However, the degree of pectin methyl-esterification did not affect the extent of Al hydrolysis. Binding of Al to purified cell wall material also resulted in Al hydrolysis in a pH- and soluble Al concentration-dependent manner, but the source of cell wall material had no effect at fixed pH. Staining of Al-treated pectin and cell wall material from wheat ( Triticum aestivum L.) and sunflower ( Helianthus annuus L.) with the Al-specific dye, chrome azurol S (CAS), resulted in the formation of a purple color, with the intensity related to the extent of Al hydrolysis.

Citation

J Bernhard Wehr, F P C Blamey, J V Hanna, P M Kopittke, G L Kerven, N W Menzies. Hydrolysis and speciation of Al bound to pectin and plant cell wall material and its reaction with the dye chrome azurol S. Journal of agricultural and food chemistry. 2010 May 12;58(9):5553-60