Restricted TRBV repertoire in CD4+ and CD8+ T-cell subsets from CML patients.

Yangqiu Li, Suxia Geng, Xin Du, Shaohua Chen, Lijian Yang, Xiuli Wu, Bo Li, Christian A Schmidt, Grzegorz K Przybylski

Institute of Hematology, Medical College, Jinan University, Guangzhou, China.

Hematology (Amsterdam, Netherlands) 2011 Jan

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T-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. In expanding our previous observations in this area, we studied the repertoire of T-cell receptor beta variable region (TRBV) and T-cell proliferative history in CD4+ and CD8+ T cells from chronic myeloid leukemia (CML) patients. The expression and clonality analysis were performed by reverse transcription-polymerase chain reaction (RT-PCR) and GeneScan technique in peripheral blood mononuclear cells (PBMCs), CD4+ and CD8+ subsets of T cells. Nineteen CML cases in chronic phase were selected for this study and 17 healthy individuals served as controls. Marked restriction of TRBV repertoire was observed in both CD4+ and CD8+ T cells from CML. In most CML samples, clonally expanded T cells were identified in CD4+ and CD8+ T cells, predominantly in TRBV19 and TRBV21 (5/19) subfamilies. In conclusion, the restricted expression of TRBV subfamilies indicates the T-cell immunodeficiency in CML patients; however, clonally expanded T cells suggest a specific immune response to leukemia associated anti gens.

Citation

Yangqiu Li, Suxia Geng, Xin Du, Shaohua Chen, Lijian Yang, Xiuli Wu, Bo Li, Christian A Schmidt, Grzegorz K Przybylski. Restricted TRBV repertoire in CD4+ and CD8+ T-cell subsets from CML patients. Hematology (Amsterdam, Netherlands). 2011 Jan;16(1):43-9