DYNLT3 is required for chromosome alignment during mouse oocyte meiotic maturation.

Xin Huang, Hai-Long Wang, Shu-Tao Qi, Zhen-Bo Wang, Jing-Shan Tong, Qing-Hua Zhang, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhong-Quan Qi, Qing-Yuan Sun

Organ Transplantation Institute, Xiamen University, Xiamen City, Fujian Province, China.

Reproductive sciences (Thousand Oaks, Calif.) 2011 Oct

filter terms:

Dynein light chain, Tctex-type 3 (DYNLT3), is a member of the cytoplasmic dynein DYNLT light chain family and has been reported to have a potential role in chromosome congression in human mitosis. However, its role in mammalian meiosis is unclear. In this study, we examined its localization, expression, and functions in mouse oocy te meiosis. Immunofluorescent staining showed that DYNLT3 was restricted to the germinal vesicle and associated with kinetochores at the germinal vesicle breakdown stage, metaphase I and metaphase II. The expression level of DYNLT3 was similar at all meiotic stages. Depletion of DYNLT3 by antibody injection resulted in chromosome misalignment and decrease of the polar body extrusion rate. We further found that DYNLT3-depleted oocytes displayed kinetochore-microtubule detachments. Chromosome-spread experiments showed that depletion of DYNLT3 inhibited the metaphase-anaphase transition by preventing homologous chromosome segregation in meiosis I. Our data suggest that DYNLT3 is required for chromosome alignment and homologous chromosome segregation during mouse oocyte meiosis.

Citation

Xin Huang, Hai-Long Wang, Shu-Tao Qi, Zhen-Bo Wang, Jing-Shan Tong, Qing-Hua Zhang, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhong-Quan Qi, Qing-Yuan Sun. DYNLT3 is required for chromosome alignment during mouse oocyte meiotic maturation. Reproductive sciences (Thousand Oaks, Calif.). 2011 Oct;18(10):983-9