Label-free fluorescence detection of the depurination activity of ribosome inactivating protein toxins.

Arun A Tanpure, Pooja Patheja, Seergazhi G Srivatsan

Indian Institute of Science Education and Research, Sai Trinity Building, Pashan, Pune 411021, India.

Chemical communications (Cambridge, England) 2012 Jan 14

filter terms:

Development of a simple label-free fluorescence hybridization assay to monitor the depurination activity of toxic ribosome inactivating proteins by using a fluorescent ligand that specifically pseudo base pairs with a cytosine residue opposite an abasic site is described. This method could be potentially implemented in screening platforms for the discovery of small molecules that inhibit the activity of these toxins. This journal is © The Royal Society of Chemistry 2012

Citation

Arun A Tanpure, Pooja Patheja, Seergazhi G Srivatsan. Label-free fluorescence detection of the depurination activity of ribosome inactivating protein toxins. Chemical communications (Cambridge, England). 2012 Jan 14;48(4):501-3