Efficient bioconversion of l-threonine to 2-oxobutyrate using whole cells of Pseudomonas stutzeri SDM.

Wen Zhang, Chao Gao, Bin Che, Cuiqing Ma, Zhaojuan Zheng, Tong Qin, Ping Xu

State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.

Bioresource technology 2012 Apr

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2-Oxobutyrate (2-OBA) is an important intermediate with many applications in the drug and chemical industries. l-Threonine, an industrial fermentation product, could be used as a suitable starting material for the 2-OBA production. In this study, whole cells of Pseudomonas stutzeri SDM were confirmed to possess a good ability to convert l-threonine into 2-OBA. The bioconversion conditions were optimized for the 2-OBA production from l-threonine. Using 9.2g dry cell weight l(-1) of whole cells of strain SDM as biocatalyst, the biocatalytic process produced 2-OBA at a high concentration (25.6gl(-1)) with a high molar conversion rate (99.6%) at 6h from 30g1(-1) of l-threonine. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

Wen Zhang, Chao Gao, Bin Che, Cuiqing Ma, Zhaojuan Zheng, Tong Qin, Ping Xu. Efficient bioconversion of l-threonine to 2-oxobutyrate using whole cells of Pseudomonas stutzeri SDM. Bioresource technology. 2012 Apr;110:719-22