Characterization of carboxylate-terminated carbosilane dendrimers and their evaluation as nanoadditives in capillary electrophoresis for vegetable protein profiling.

Protein profiles are becoming an important tool to differentiate and classify varieties of several cultivars and to obtain a specific fingerprint for them. The use of protein profiles for these purposes needs to achieve high separation efficiencies to obtain a high number of well resolved peaks. In this work, carbosilane dendrimers with interior carbon-silicon bonds and negatively charged in the dendrimer surface with carboxylic acid as functional groups were employed as nanoadditives to separate soybean and olive seeds proteins. First, these dendrimers were characterized using CE to evaluate their possible impurities. A potentiometric titration was later carried out to determine their pK(a) values. Afterwards, the characterized dendrimers were used to improve the protein profiles obtained by EKC for vegetable proteins. Different dendrimer generations (G1, G2, and G3) and concentrations (0.01-1% m/v) were tested. The highest dendrimer generation G3 at 0.1% (m/v) allowed observing the best protein profiles for soybean and olive seeds. These results demonstrate that carboxylate-terminated carbosilane dendrimers are attractive nanoadditives in EKC for the effective separation of vegetable proteins. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

C Montealegre, B Rasines, R Gómez, F J de la Mata, C García-Ruiz, M L Marina. Characterization of carboxylate-terminated carbosilane dendrimers and their evaluation as nanoadditives in capillary electrophoresis for vegetable protein profiling. Journal of chromatography. A. 2012 Apr 20;1234:16-21

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PMID: 22429548

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