The β-amylase polymorphism of winter common wheat grains].

V P Netsvetaev, O V Akinshina, L S Bondarenko, I P Motorina

Genetika 2012 Feb

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The polymorphism of winter common wheat with respect to β-amylase isoenzymes has been analyzed using electrophoresis in polyacrylamide gel (PAAG) buffered with a Tris-glycine system (pH 8.3). Seven β-amylase isoenzymes have been found in wheat cultivars and the breeding stock. Isoenzymes A, B, and C are the most frequent in Russian and Ukrainian cultivars (51.7 4.7, 30.7 3.8, and 11.9 2.5%, respectively). Two alleles of the β-Amy-D1 locus of the long arm of chromosome 4D have been identified. The substrate-enzyme affine effect can be used to locate the zones of activity of this enzyme by means of staining for proteins. It has been determined that β-amylase zymotypes may play a role in the aggregating capacity of the grain protein complex via the formation of S-S bonds.

Citation

V P Netsvetaev, O V Akinshina, L S Bondarenko, I P Motorina. The β-amylase polymorphism of winter common wheat grains]. Genetika. 2012 Feb;48(2):168-74