Junfen Li, Baohong Li, Yanbo Wu, Shaomin Shuang, Chuan Dong, Martin M F Choi
School of Chemistry and Chemical Engineering, Research Center of Environmental Science and Engineering, Shanxi University, 92 Wucheng Street, Taiyuan 030006, PR China.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2012 SepThe binding mode and mechanism of the interactions between two planar cationic alkaloids chelerythrine (Che) and sanguinarine (San) with calf thymus DNA (ctDNA) were systematically investigated at pH 5.40 using UV-vis absorption spectroscopy, fluorescence spectroscopy and cyclic voltammetry. Che and San show strong fluorescence at 570 and 589 nm, respectively. Che displays fluorescence enhancement with ctDNA whereas the fluorescence of San is quenched on interaction with ctDNA. In addition, UV-vis spectra of both alkaloids show apparent hypochromicity and are bathochromic shifted, indicating that they could intercalate into ctDNA bases. The fluorescence polarization of Che and San increases in the presence of ctDNA, again implying the intercalation of two alkaloids with ctDNA. This conclusion was also supported by the results obtained from anion quenching and cyclic voltammetry. The binding constants of both alkaloids with ctDNA were calculated in the order of 10(5)L/mol. San binds with ctDNA 3-fold stronger than Che. The stoichiometric bindings are five nucleotides per Che or San. Electrostatic binding also exists between the alkaloids and DNA helix. Finally, theoretical calculations show that only certain parts of Che and San molecules intercalate into the DNA helix. Copyright © 2012 Elsevier B.V. All rights reserved.
Junfen Li, Baohong Li, Yanbo Wu, Shaomin Shuang, Chuan Dong, Martin M F Choi. Luminescence and binding properties of two isoquinoline alkaloids chelerythrine and sanguinarine with ctDNA. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2012 Sep;95:80-5
PMID: 22613127
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