Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of ade1 or ade2 mutants.

Marina G Tarutina, Tatiana A Dutova, Inna E Yezhova, Hiroaki Nishiuchi, Sergey P Sineoky

Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhny proezd 1, 117545 Moscow, Russia.

Journal of bioscience and bioengineering 2012 Dec

filter terms:

We identified Saccharomyces cerevisiae mutants with 100% higher intracellular glutathione using 1-methyl-3-nitro-1-nitrosoguanidine mutagenesis. This method employs visual selection of the most pigmented colonies among met30 strains carrying ade1 and ade2 mutations. Since the method does not involve genetic engineering, the mutants are suitable for use in the food industry. Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Citation

Marina G Tarutina, Tatiana A Dutova, Inna E Yezhova, Hiroaki Nishiuchi, Sergey P Sineoky. Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of ade1 or ade2 mutants. Journal of bioscience and bioengineering. 2012 Dec;114(6):615-8