Potent inhibition of human neutrophil activations by bractelactone, a novel chalcone from Fissistigma bracteolatum.

Yang-Chang Wu, Munisamy Sureshbabu, Yao-Ching Fang, Yi-Hsiu Wu, Yu-Hsuan Lan, Fang-Rong Chang, Ya-Wen Chang, Tsong-Long Hwang

Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan.

Toxicology and applied pharmacology 2013 Feb 1

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Fissistigma bracteolatum is widely used in traditional medicine to treat inflammatory diseases. However, its active components and mechanisms of action remain unclear. In this study, (3Z)-6,7-dihydroxy-4-methoxy-3-(phenylmethylidene)-5-(3-phenylpropanoyl)-1-benzofuran-2(3H) (bractelactone), a novel chalcone from F. bracteolatum, showed potent inhibitory effects against superoxide anion (O₂·⁻) production, elastase release, and CD11b expression in formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP)-induced human neutrophils. However, bractelactone showed only weak inhibition of phorbol myristate acetate-caused O₂·⁻ production. The peak cytosolic calcium concentration ([Ca²⁺](i)) was unaltered by bractelactone in FMLP-induced neutrophils, but the decay time of [Ca²⁺](i) was significantly shortened. In a calcium-free solution, changes in [Ca²⁺](i) caused by the addition of extracellular Ca²⁺ were inhibited by bractelactone in FMLP-activated cells. In addition, bractelactone did not alter the phosphorylation of p38 MAPK, ERK, JNK, or AKT or the concentration of cAMP. These results suggest that bractelactone selectively inhibits store-operated calcium entry (SOCE). In agreement with this concept, bractelactone suppressed sustained [Ca²⁺](i) changes in thapsigargin-activated neutrophils. Furthermore, bractelactone did not alter FMLP-induced formation of inositol 1,4,5-triphosphate. Taken together, our results demonstrate that the anti-inflammatory effects of bractelactone, an active ingredient of F. bracteolatum, in human neutrophils are through the selective inhibition of SOCE. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Yang-Chang Wu, Munisamy Sureshbabu, Yao-Ching Fang, Yi-Hsiu Wu, Yu-Hsuan Lan, Fang-Rong Chang, Ya-Wen Chang, Tsong-Long Hwang. Potent inhibition of human neutrophil activations by bractelactone, a novel chalcone from Fissistigma bracteolatum. Toxicology and applied pharmacology. 2013 Feb 1;266(3):399-407