Construction of a marker system for the evaluation of competitiveness for legume nodulation in Rhizobium strains.

Carmen Sánchez-Cañizares, Jose Palacios

Journal of microbiological methods 2013 Mar

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A marker system has been set up for the analysis of competitiveness of Rhizobium leguminosarum strains for legume nodulation. The strains generated incorporate gusA and celB marker genes at identical positions and allow efficient scoring of single and double infections. Based on this system, we have found that strain UPM791 outcompetes 3841 for nodulation in pea. This technique will be useful to determine the effect of different traits on competitiveness. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Carmen Sánchez-Cañizares, Jose Palacios. Construction of a marker system for the evaluation of competitiveness for legume nodulation in Rhizobium strains. Journal of microbiological methods. 2013 Mar;92(3):246-9