Post-injury conditioning with lipopolysaccharide or lipooligosaccharide reduces inflammation in the brain.

Deborah Bingham, Constance M John, Jack Levin, S Scott Panter, Gary A Jarvis

Center for Immunochemistry, 4150 Clement Street, Veterans Affairs Medical Center, San Francisco, CA 94121, USA.

Journal of neuroimmunology 2013 Mar 15

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Traumatic brain injury (TBI) is a leading cause of mortality and disability in the Western world. The first stage of TBI results from the mechanical damage from an impact or blast. A second stage occurs as an inflammatory response to the primary injury and presents an opportunity for clinical intervention. In this study, we investigated the effect of pre- and post-injury treatment with lipopolysaccharide (LPS) from Escherichia coli and lipooligosaccharide (LOS) from Neisseria meningitidis on levels of cerebral inflammatory cells, circulating blood cells, and pro- and anti-inflammatory cytokine levels in a rat model of neuroinflammation induced by intrastriatal injection of IL-1β to mimic the second stage of TBI. LPS or LOS was administered intravenously (IV) or intranasally (IN) 2h pre- or post-injection of IL-1β. The rats were euthanized 12h following IL-1β injection. Brain sections were immunostained with antibody to ED-1, a microglia cell marker. Cells in whole blood were assessed with a VetScan HM2 analyzer, and cytokine levels in sera were analyzed with a Bio-Plex system. Pre- and post-injury IV administration of LPS or LOS significantly reduced microglia in the brain, and IN pre-treatment with LPS or LOS showed a statistical trend towards reducing microglia. Pre- and post-treatment IV with LOS increased circulating levels of IL-2 and IL-4, whereas IN post-treatment with LPS reduced levels of the inflammatory cytokines, TNF-α and IFN-γ. The findings strongly support continued investigation of post-conditioning with LPS or LOS as potential neuroprotective treatments for neuroinflammation from TBI. Published by Elsevier B.V.

Citation

Deborah Bingham, Constance M John, Jack Levin, S Scott Panter, Gary A Jarvis. Post-injury conditioning with lipopolysaccharide or lipooligosaccharide reduces inflammation in the brain. Journal of neuroimmunology. 2013 Mar 15;256(1-2):28-37