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In the present study, mutant strain of Klebsiella pneumoniae with deletion of the crr gene encoding EIIA(Glc) (a component of the glucose-specific phosphoenolpyruvate-dependent transferase system [PTS]) was prepared. This eliminated the ability of the strain to mediate carbon catabolite repression (CCR). Production of 1,3-propanediol (1,3-PD) from glycerol by the crr mutant strain was enhanced (compared to that of the parent) in the presence of glucose. Using molasses as a co-substrate of glycerol, the maximum yield of 1,3-PD was 60.4% greater (81.2g/l) than that obtained when glycerol was used alone, under optimum fermentation conditions. Copyright © 2012 Elsevier Ltd. All rights reserved.


Baek-Rock Oh, Won-Kyung Hong, Sun-Yeon Heo, Lian Hua Luo, Akihiko Kondo, Jeong-Woo Seo, Chul Ho Kim. The production of 1,3-propanediol from mixtures of glycerol and glucose by a Klebsiella pneumoniae mutant deficient in carbon catabolite repression. Bioresource technology. 2013 Feb;130:719-24

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PMID: 23334032

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