Display of Clostridium cellulovorans xylose isomerase on the cell surface of Saccharomyces cerevisiae and its direct application to xylose fermentation.

Miki Ota, Hiroshi Sakuragi, Hironobu Morisaka, Kouichi Kuroda, Hideo Miyake, Yutaka Tamaru, Mitsuyoshi Ueda

Div. of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, 606-8502, Japan.

Biotechnology progress 2013 Mar-Apr

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Xylose isomerase (XI) is a key enzyme in the conversion of D-xylose, which is a major component of lignocellulosic biomass, to D-xylulose. Genomic analysis of the bacterium Clostridium cellulovorans revealed the presence of XI-related genes. In this study, XI derived from C. cellulovorans was produced and displayed using the yeast cell-surface display system, and the xylose assimilation and fermentation properties of this XI-displaying yeast were examined. XI-displaying yeast grew well in medium containing xylose as the sole carbon source and directly produced ethanol from xylose under anaerobic conditions. Copyright © 2013 American Institute of Chemical Engineers.

Citation

Miki Ota, Hiroshi Sakuragi, Hironobu Morisaka, Kouichi Kuroda, Hideo Miyake, Yutaka Tamaru, Mitsuyoshi Ueda. Display of Clostridium cellulovorans xylose isomerase on the cell surface of Saccharomyces cerevisiae and its direct application to xylose fermentation. Biotechnology progress. 2013 Mar-Apr;29(2):346-51