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An immobilization method using net was developed for fumaric acid fermentation by Rhizopus arrhizus RH-07-13. The large surface of the net immobilized enough filamentous mycelia which produced fumaric acid rapidly. Net size and spore concentration were optimized to enhance fermentation performance and 150cm(2) of net size, 0.5×10(6)per ml of spore concentration were selected finally. Compared to free-cell fermentation, fumaric acid production was flat (32.03 vs. 31.23g/L) but fermentation time reduced 83.3% (24 vs. 144h). Copyright © 2012 Elsevier Ltd. All rights reserved.


Chunbo Gu, Yuqing Zhou, Luo Liu, Tianwei Tan, Li Deng. Production of fumaric acid by immobilized Rhizopus arrhizus on net. Bioresource technology. 2013 Mar;131:303-7

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PMID: 23360706

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