High throughput LC-MS/MS method for simultaneous determination of zidovudine, lamivudine and nevirapine in human plasma.

Valluru Rajani Kumar, B Phani Bhushana Reddy, B Ravi Kumar, K Sreekanth, Kilaru Naveen Babu

Department of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, India; Axis Clinicals Limited, 1-121/1, Miyapur, Hyderabad 500 049, India.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2013 Mar 15

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A selective and sensitive high performance liquid chromatography-tandem mass spectrometry method has been developed and validated for simultaneous determination of zidovudine (ZDV), lamivudine (3TC) and nevirapine (NVP) in human plasma. After Solid phase extraction (SPE), analytes and ISTDs were run on Peerless Basic C18 column with an injection volume of 3μL and run time of 3.0min. An isocratic mobile phase of 0.1% formic acid in water:methanol (15:85, v/v) was used with positive mass spectrometric detection. The method was validated over a concentration range of 5-1500ng/mL for ZDV and 3TC and over the concentration range of 10-3000ng/mL for NVP. The intraday and interday precision and accuracy across four validation runs were ranged from 1.6 to 10.1% and 93.8 to 110.8% respectively. Copyright © 2013 Elsevier B.V. All rights reserved.

Citation

Valluru Rajani Kumar, B Phani Bhushana Reddy, B Ravi Kumar, K Sreekanth, Kilaru Naveen Babu. High throughput LC-MS/MS method for simultaneous determination of zidovudine, lamivudine and nevirapine in human plasma. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2013 Mar 15;921-922:9-14