Characterization of feline TRIM genes: molecular cloning, expression in tissues, and response to type I interferon.

Ryota Koba, Chika Kokaji, Gentoku Fujisaki, Keisuke Oguma, Hiroshi Sentsui

Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-8510, Japan.

Veterinary immunology and immunopathology 2013 May 15

filter terms:

Members of the tripartite motif (TRIM) protein family in mammals are responsible for various cellular processes. Previous studies have revealed that several TRIM proteins were induced by interferons (IFN) and that these proteins were involved in innate immune response against retroviral infection. Although retroviral infection is prevalent in domestic cats, the expression profiles and roles of feline TRIM genes against these viral infections are not well understood. In the present study, we examined tissue expression and IFN inducibility of nine feline TRIM genes. In addition, the complete coding sequences of six cloned TRIM genes were determined, and their structures were analyzed. Nine TRIM genes were expressed in feline tissues and five were up-regulated by type I IFN. The predicted amino acid sequence of six feline TRIM proteins showed high sequence similarities to other mammalian TRIM proteins, and suggest that feline TRIM genes are potentially involved in antiviral reactivity in IFN-mediated immune response. Copyright © 2013 Elsevier B.V. All rights reserved.

Citation

Ryota Koba, Chika Kokaji, Gentoku Fujisaki, Keisuke Oguma, Hiroshi Sentsui. Characterization of feline TRIM genes: molecular cloning, expression in tissues, and response to type I interferon. Veterinary immunology and immunopathology. 2013 May 15;153(1-2):91-8