First description of polytene chromosomes in biting midges (Diptera: Ceratopogonidae).

Polytene chromosomes are described from secretory cells in larvae of Forcipomyia nigra (Winnertz). They are present in large glandular-trichogen cells at the bases of secretory setae and in midgut cells that were observed by transmission electron microscopy and light microscopy. Polytene chromosomes, isolated from the glandular-trichogen cells using aceto-orcein squash technique, measure 50-200 microm, have braid-like strands of chromatin and no bands, features that are unique within the Culicomorpha.

Citation

Aleksandra Urbanek, Ryszard Szadziewski, Wojciech Giłka, Art Borkent. First description of polytene chromosomes in biting midges (Diptera: Ceratopogonidae). Journal of medical entomology. 2013 Mar;50(2):458-61

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PMID: 23540136

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