Induction of UGT1A1 expression by praeruptorin A and praeruptorin C through hCAR pathway].

This study is purposed to investigate the effects of praeruptorin A (PA) and praeruptorin C (PC) on UGT1A1 in HepG2 cells through hCAR pathway. PA and PC were incubated with HepG2 cells for 24 h and 48 h, mRNA and protein expressions of UGT1A1 were determined by real-time PCR and Western blotting assays. Additionally, effects of PA and PC on UGT1A1 mRNA and protein expressions were also measured after transient transfection of a specific CAR siRNA for 72 h in HepG2 cells. UGT1A1 mRNA and protein expression levels were significantly increased by PA and PC after incubation for 48 h. Moreover, the mRNA and protein up-regulations of UGT1A1 were attenuated by transient transfection of a specific CAR siRNA, suggesting the induction was mediated by CAR. The results suggest that PA and PC can significantly up-regulate UGT1A1 expression partially via the CAR-mediated pathway.

Citation

Xu-Nian Zhou, Hui-Chang Bi, Jing Jin, Rong-Rong Deng, Meng-Jia Ying, Yong-Tao Wang, Min Huang. Induction of UGT1A1 expression by praeruptorin A and praeruptorin C through hCAR pathway]. Yao xue xue bao = Acta pharmaceutica Sinica. 2013 May;48(5):794-8

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PMID: 23888707

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