Inflammasome-independent IL-1β mediates autoinflammatory disease in Pstpip2-deficient mice.

Proceedings of the National Academy of Sciences of the United States of America 2014 Jan 21

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Chronic recurrent multifocal osteomyelitis (CRMO) is a human autoinflammatory disorder that primarily affects bone. Missense mutation (L98P) of proline-serine-threonine phosphatase-interacting protein 2 (Pstpip2) in mice leads to a disease that is phenotypically similar to CRMO called chronic multifocal osteomyelitis (cmo). Here we show that deficiency of IL-1RI in cmo mice resulted in a significant reduction in the time to onset of disease as well as the degree of bone pathology. Additionally, the proinflammatory cytokine IL-1β, but not IL-1α, played a critical role in the pathology observed in cmo mice. In contrast, disease in cmo mice was found to be independent of the nucleotide-binding domain, leucine-rich repeat-containing family, pyrin domain-containing 3 (NLRP3) inflammasome as well as caspase-1. Neutrophils, but not bone marrow-derived macrophages, from cmo mice secreted increased IL-1β in response to ATP, silica, and Pseudomonas aeruginosa compared with neutrophils from WT mice. This aberrant neutrophil response was sensitive to inhibition by serine protease inhibitors. These results demonstrate an inflammasome-independent role for IL-1β in disease progression of cmo and implicate neutrophils and neutrophil serine proteases in disease pathogenesis. These data provide a rationale for directly targeting IL-1RI or IL-1β as a therapeutic strategy in CRMO.

Citation

Suzanne L Cassel, John R Janczy, Xinyu Bing, Shruti P Wilson, Alicia K Olivier, Jesse E Otero, Yoichiro Iwakura, Dmitry M Shayakhmetov, Alexander G Bassuk, Yousef Abu-Amer, Kim A Brogden, Trudy L Burns, Fayyaz S Sutterwala, Polly J Ferguson. Inflammasome-independent IL-1β mediates autoinflammatory disease in Pstpip2-deficient mice. Proceedings of the National Academy of Sciences of the United States of America. 2014 Jan 21;111(3):1072-7