Src kinase inhibitor PP2 protects rat astrocytes from hypoxia/reoxygenation injury in vitro].

To investigate the effect of Src kinase inhibitor PP2 on hypoxia/reoxygenation (H/R) injury in rat astrocytes in vitro. In vitro cultured rat astrocytes were exposed to hypoxia for 8 h followed by reoxygenation for 24 h with or without pretreatment with PP2 (10 µmol/L) for 24 h before H/R injury. MTT assay and flow cytometry were used to detect the viability and apoptosis of the exposed astrocytes, respectively, and the protein expressions of Src, Bax, and Bcl-2 in the cells were determined using Western blotting. PP2 pretreatment significantly increased the viability and decreased the apoptosis rate of rat astrocytes exposed to H/R injury (P<0.01). Western blotting showed that H/R injury caused increased expression of Src kinase, which was lowered by PP2 pretreatment. The ratio of Bax/bcl-2 in the astrocytes increased after H/R injury, and was significantly decreased by PP2 pretreatment (P<0.01). PP2 protects rat astrocytes from H/R injury possibly by inhibiting the expression of Src kinase and activating the anti-apoptotic mechanisms in the cells.

Citation

Yuchen Gu, Xuhui Tong, Li Yu, Hao Jiao, Binbin Yu, Shuying Dong. Src kinase inhibitor PP2 protects rat astrocytes from hypoxia/reoxygenation injury in vitro]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 2015 Feb;35(2):239-43

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PMID: 25736120

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