Investigations into the binding of jadomycin DS to human topoisomerase IIβ by WaterLOGSY NMR spectroscopy.

Camilo F Martinez-Farina, Nicole McCormick, Andrew W Robertson, Helen Clement, Alison Jee, Anna Ampaw, Nei-Li Chan, Ray T Syvitski, David L Jakeman

Organic & biomolecular chemistry 2015 Nov 7

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The jadomycins are a family of secondary metabolites produced by S. venezuelae ISP5230. Specific jadomycins have been shown to possess a variety of anticancer, antifungal, and antibacterial properties, with different molecular mechanisms of action. Herein we demonstrate qualitative and quantitative direct binding between the validated anticancer target human topoisomerase IIβ and jadomycin DS using WaterLOGSY NMR spectroscopy. Additionally, we report for the first time, that jadomycin DS also binds a variety of other proteins, likely in a non-specific manner. Such interactions may rationalize the potential polypharmacology of jadomycin DS.

Citation

Camilo F Martinez-Farina, Nicole McCormick, Andrew W Robertson, Helen Clement, Alison Jee, Anna Ampaw, Nei-Li Chan, Ray T Syvitski, David L Jakeman. Investigations into the binding of jadomycin DS to human topoisomerase IIβ by WaterLOGSY NMR spectroscopy. Organic & biomolecular chemistry. 2015 Nov 7;13(41):10324-7