Correlation Engine 2.0
Clear Search sequence regions


  • 4 hydroxytamoxifen (2)
  • aryl hydrocarbon hydroxylases (2)
  • breast cancer (1)
  • cells (2)
  • CYP1a1 (3)
  • CYP1A2 (1)
  • CYP2b2 (1)
  • CYP2c11 (1)
  • CYP2C9 (1)
  • CYP2d4 (1)
  • cyp2d6 (3)
  • CYP2E1 (1)
  • CYP3A (2)
  • CYP3a2 (1)
  • did (1)
  • female (1)
  • half life (1)
  • Hep (1)
  • human (3)
  • hydroxytamoxifen (1)
  • isoforms (3)
  • liver (3)
  • minor (1)
  • mrna (2)
  • osteoporosis (1)
  • P 450 (6)
  • patients (1)
  • plant extracts (2)
  • rats (6)
  • rna (2)
  • ROC (1)
  • steroid (1)
  • steroid hydroxylases (2)
  • tamoxifen (9)
  • trifolium (10)
  • Sizes of these terms reflect their relevance to your search.

    Tamoxifen is used in clinical practice for breast cancer patients and to prevent osteoporosis. Red clover (Trifolium pratense) preparations are consumed worldwide as dietary supplements for relieving postmenopausal symptoms. In the present study we investigated the possible herb-drug interaction between red clover and tamoxifen in rats. 15 days pre-treatment with red clover did not alter the tamoxifen and its active metabolite 4-hydroxytamoxifen pharmacokinetics significantly (pā€‰>ā€‰0.05). Therefore the therapeutic efficacy of the tamoxifen may not be compromised by the co-administration with red clover. Tamoxifen metabolism is primarily mediated by CYP2D6, CYP3A4 with minor contribution from CYP2C9, CYP2E1 and CYP1A2 isoforms. Although, red clover pre-treatment significantly (pā€‰<ā€‰0.05) decreased the mRNA expression and activity of CYP3a2, no effect on CYP2d4 and increased expression and activity of CYP2c11 could be the plausible reasons for lack of effect on tamoxifen and its metabolite pharmacokinetics in rats. CYP1a1 and CYP2b2 mRNA expression and activity were also significantly reduced by red clover. To extend the clinical utility of the present study, effect of red clover extract on major CYPs using human liver microsomes and HepG2 cell lines were also determined. Similar finding were observed in the human liver preparations as in rats.

    Citation

    Kanumuri Siva Rama Raju, Isha Taneja, Guru Raghavendra Valicherla, Murali Krishna Challagundla, Mamunur Rashid, Anees Ahmed Syed, Jiaur Rahman Gayen, Sheelendra Pratap Singh, Muhammad Wahajuddin. No effect on pharmacokinetics of tamoxifen and 4-hydroxytamoxifen by multiple doses of red clover capsule in rats. Scientific reports. 2015;5:16126

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 26530625

    View Full Text