Cysteine proteinase-inhibitory activity of ras gene product is not affected by mutations at GTPase-activating protein-binding sites.

T Hiwasa, T Sawada, S Sakiyama, E Kominami, N Katunuma

Division of Biochemistry, Chiba Cancer Center Research Institute, Japan.

Biological chemistry Hoppe-Seyler 1989 Nov

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We have investigated the proteinase-inhibitory activity against cathepsin L of some c-Ha-ras gene products (p21s) with point mutations at position 12, 33 or 35, which affect the interaction with GTPase-activating protein (GAP). All of the full-length p21s examined showed similar inhibitory activities irrespective of the point mutation and the transforming activity. These results suggest that mutations at GAP-interacting sites have no effect on the proteinase-inhibitory activity of p21s and that the proteinase-inhibitory activity alone is not sufficient for the transformation caused by p21s.

Citation

T Hiwasa, T Sawada, S Sakiyama, E Kominami, N Katunuma. Cysteine proteinase-inhibitory activity of ras gene product is not affected by mutations at GTPase-activating protein-binding sites. Biological chemistry Hoppe-Seyler. 1989 Nov;370(11):1215-20