Liang Zhu, Maohong Cao, Yaohui Ni, Lijian Han, Aihua Dai, Rongrong Chen, Xiaojin Ning, Xiaorong Liu, Kaifu Ke
Cellular and molecular neurobiology 2016 Jun 28Human transforming growth factor β-activated kinase (TAK1)-binding protein 3 (TAB3) is a regulator of NF-κB which has been mainly found in a variety of cancers. While TAB3 is highly expressed in brain tissue, little is known about the function of TAB3 in central nervous system. Our group established an animal ICH model with autologous whole blood injected into brain, and also a cell ICH model with hemin stimulation. Our Western blot result showed up-regulation of TAB3 during neuronal apoptosis in the model of intracerebral hemorrhage (ICH), which was also approved by immunofluorescence and immunohistochemistry result. Besides, increasing TAB3 level was accompanied by the increased expression of active-caspase-3, active-caspase-8, and decreased expression of Bcl-2. Furthermore, in in vitro study, the level of neuronal apoptosis was decreased by applying TAB3- RNA interference in PC12 cells. All the results above suggested that TAB3 probably participates in the process of neuronal apoptosis following ICH.
Liang Zhu, Maohong Cao, Yaohui Ni, Lijian Han, Aihua Dai, Rongrong Chen, Xiaojin Ning, Xiaorong Liu, Kaifu Ke. Up-Regulation of TAB3 Is Involved in Neuronal Apoptosis After Intracerebral Hemorrhage. Cellular and molecular neurobiology. 2016 Jun 28
PMID: 27352012
View Full Text