Transmitting characters of individual E chromosomes of Thinopyrum elongatum in Triticum turgidum background.

The transmission patterns of Thinopyrum elongatum chromosomes in the background of Triticum turgidum were investigated through cytogenetic and molecular marker analysis based on the F2 and F3 plants derived from a cross between Triticum trititrigia (AABBEE) and T. turgidum L. ssp. durum (AABB). An additional objective was to develop durum-Th. elongatum E chromosome addition lines. Among 218 F2 plants, individuals with 2n=28 accounted for 41.7%, those with 2n=29 accounted for 18.3%, and the remaining 40.0% had 2n=31-42. Molecular marker analysis of the F2 monosomic addition plants with 2n=29 showed significant differences in transmission rate among Th. elongatum chromosomes. Chromosome 1E had the highest transmission rates, while 3E and 6E had the lowest. Among F2 double monosomic addition plants with 2n=30, joint transmission was frequent between 1E, 4E, 7E and 5E; 6E was less frequent in joint transmission with other chromosomes, while 2E and 3E never transmitted along with other chromosomes. Among F3 plants derived from F2 monosomic addition plants, the transmission rates of E chromosomes varied widely, from the minimum of 9.1% for 6E to the maximum of 27.5% for 1E, consistent with observations from F2 plants. A full set of durum-Th. elongatum 1E-7E monosomic addition lines and several disomic addition lines were selected from F3 plants, and all monosomic addition plants were fertile. These chromosome E addition lines will be useful intermediate materials for developing substitution lines and translocation lines.

Citation

Hai-feng Li, Hui-ping Liu, Yi Dai, Shuai Huang, Jun Zhang, Yong Gao, Jianmin Chen. Transmitting characters of individual E chromosomes of Thinopyrum elongatum in Triticum turgidum background. Yi chuan = Hereditas. 2016 Nov 20;38(11):1020-1029

Mesh Tags

PMID: 27867152

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