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To knock out PDK1 by using Vav-Cre and observe the effects of PDK1 knock out on the ratio, number and differentiation of neutrophil. The PDK1 expression level of Vav-Cre;PDK1lox/lox mouse in the bone marrow cells was analyzed by RT-PCR. The effect of PDK1 on hematopoietic progenitor was observed by CFU-C assay, and the effect of PDK1 on the ratio and number of neutrophil was detected by flow cytometric analysis. The RNA expression level of bone marrow cells of Vav-Cre;PDK1lox/lox mouse was dramatically lower than that of the control mouse. The number of functional GMP was lower in Vav-Cre;PDK1lox/lox mouse in contrast to controls. The percentage and number of neutrophil were lower, but the percentage of premyelocyte/myelocytes was more than 2 times in Vav-Cre;PDK1lox/lox group compared with control groups. PDK1 affects the process of the differention of hematopoietic stem cells to GMP, the neutrophil differention and maturation.

Citation

Wei-Li Wang, Le Wang, Tian-Yuan Hu, Cong Li, Wei-Ping Yuan. Effect of PDK1 on Neutrophil Differentiation]. Zhongguo shi yan xue ye xue za zhi. 2017 Jun;25(3):932-935


PMID: 28641662

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