Clear Search sequence regions


  • 8 br- cgmp (2)
  • apoptosis (5)
  • Bcl 2 (1)
  • Cgmp (8)
  • CNP (17)
  • control group (4)
  • cTn (2)
  • cyclic (2)
  • cyclic gmp (2)
  • gene knockdown (3)
  • heart (1)
  • IL 6 (2)
  • ischemia (2)
  • LDH (2)
  • NPRB (5)
  • protein rat (1)
  • rats (6)
  • receptors factor (3)
  • serum (1)
  • signal (1)
  • TNF α (2)
  • tunel (1)
  • Sizes of these terms reflect their relevance to your search.

    This study aimed to explore effects of CNP on ventricular remodeling following myocardial ischemia-reperfusion (I/R) injury through the NPRB/cGMP signaling pathway. Rat cardiomyocytes were assigned into: control, I/R, I/R + CNP, and I/R + 8-Br-cGMP groups. ELISA, qRT-PCR, and Western blotting were used to detect cGMP content and expression, respectively. After model establishment of I/R rats, normal control, CNP-/- control, I/R, and CNP-/- groups were set. Indexes of heart were detected using echocardiography and hemodynamics. ELISA was used to measure serum CNP, cGMP, LDH, cTn I, CK-MB, TNF-α, and IL-6 levels. Myocardial infarct was identified by TTC staining, and apoptosis conditions by TUNEL staining. QRT-PCR and Western blotting were adopted to detect expressions of CNP, NPRB, cGMP, and apoptosis-related genes. Compared with control group, cGMP contents and expression in the I/R, I/R + CNP and I/R + 8-Br-cGMP groups were decreased. Levels of LVEDV, LVESV, LVDS, LVDD, IVSD, LVM, LVEDP, and LVSP were higher in the I/R, CNP-/- control, and CNP-/- groups than normal control group while LVEF, SV, CO, and ±dp/dtmax were lower. Compared with the normal control group, LDH, cTn I, CK-MB, TNF-α, and IL-6 were higher in the I/R, CNP-/- control and CNP-/- groups; pathological changes and myocardial infarction were observed in the I/R, CNP-/- control, and CNP-/- groups; expressions of apoptosis-related genes in those groups were higher; while CNP, NPRB, cGMP, and Bcl-2 expressions were decreased. We came to the conclusion that gene knockdown of CNP blocks the NPRB/cGMP signaling pathway, thereby aggravating myocardial I/R injury and causing ventricular remodeling in rats. © 2017 Wiley Periodicals, Inc.

    Citation

    Lian-He Wu, Qi Zhang, Shen Zhang, Lu-Yu Meng, Yan-Chi Wang, Cun-Jian Sheng. Effects of gene knockdown of CNP on ventricular remodeling after myocardial ischemia-reperfusion injury through NPRB/Cgmp signaling pathway in rats. Journal of cellular biochemistry. 2018 Feb;119(2):1804-1818

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 28796407

    View Full Text