In Vitro Antileukemic Activity of Formamidine Epidoxorubicin Analogs.

Epidoxorubicin is an anthracycline agent. The present study was undertaken to compare the antileukemic potential of epidoxorubicin and its two formamidine analogs containing either a morpholine moiety (EPIFmor) or a hexamethyleneimine moiety (EPIFhex) in the amidine group. The experiments were performed in vitro on MOLT-4 cells using spectrophotometry, Coulter electrical impedance, flow cytometry, and light microscopy methods. The leukemia cell responses to the action of the anthracyclines were manifested in their different viability, count and volume, degree of apoptosis and necrosis, activity of caspases -8, -9, and -3/7, mitochondrial membrane potential, and in the cell-cycle distribution. In general, epidoxorubicin appeared to be the most active, and EPIFmor was more active than EPIFhex against MOLT-4 cells. The structural modifications of epidoxorubicin in the amidine group were responsible for the varied action of its formamidine analogs on human acute lymphoblastic leukemia cells. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Citation

Lidia Mazur, Małgorzata Opydo-Chanek, Katarzyna Śladowska, Barbara Janota, Urszula Kłaput, Małgorzata Łukawska, Irena Oszczapowicz. In Vitro Antileukemic Activity of Formamidine Epidoxorubicin Analogs. Anticancer research. 2017 Nov;37(11):6363-6372

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PMID: 29061821

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