Accuracy of in-house alcohol-dissolved wheat extract for diagnosing IgE-mediated wheat allergy.

Punchama Pacharn, Nunthana Siripipattanamongkol, Nittida Pannakapitak, Nualanong Visitsunthorn, Orathai Jirapongsananuruk, Surapon Piboonpocanun, Pakit Vichyanond

Asian Pacific journal of allergy and immunology 2020 Jun

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The standard method for diagnosing immediate wheat allergy is oral food challenge test (OFC). However, OFC can provoke anaphylaxis during the challenge process. Skin prick test (SPT) using commercial wheat extract yielded unsatisfactory result for diagnosis of wheat allergy. As a result, an in-house, alcohol-dissolved (Coca-10% EtOH) wheat extract was developed to improve accuracy of the SPT. To determine the accuracy of in-house, alcohol-dissolved wheat extract in children with immediate wheat allergy. This prospective cross-sectional study included children with history of immediate reaction after wheat ingestion. SPTs with commercial and in-house Coca-10% EtOH wheat extract were performed and wheat and omega-5 (ω-5) gliadin specific IgE (sIgE) were measured. Patients with no history of recent anaphylaxis after wheat ingestion underwent OFC with 31 grams of wheat flour. Thirty children were recruited. Thirteen of those had history of anaphylaxis after wheat ingestion. Eleven of the remaining 17 children (64.7%) had a positive result for wheat challenge test. Wheal size of 3 mm for both in-house and commercial wheat extract yielded the best accuracy for the test. Using these cutoff parameters, in-house Coca-10% EtOH wheat extract yielded 91.7% sensitivity, 66.7% specificity, and 86.7% accuracy. Comparatively, the commercial extract yielded 70.8% sensitivity, 100% specificity, and 76.6% accuracy. SPT using in-house Coca-10% EtOH wheat extract yielded better accuracy than commercial extract for diagnosing immediate type wheat allergy in children.

Citation

Punchama Pacharn, Nunthana Siripipattanamongkol, Nittida Pannakapitak, Nualanong Visitsunthorn, Orathai Jirapongsananuruk, Surapon Piboonpocanun, Pakit Vichyanond. Accuracy of in-house alcohol-dissolved wheat extract for diagnosing IgE-mediated wheat allergy. Asian Pacific journal of allergy and immunology. 2020 Jun;38(2):102-107