Distinct Binding Preferences between Ras and Raf Family Members and the Impact on Oncogenic Ras Signaling.

Elizabeth M Terrell, David E Durrant, Daniel A Ritt, Nancy E Sealover, Erin Sheffels, Russell Spencer-Smith, Dominic Esposito, Yong Zhou, John F Hancock, Robert L Kortum, Deborah K Morrison

Molecular cell 2019 Dec 19

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The Ras GTPases are frequently mutated in human cancer, and, although the Raf kinases are essential effectors of Ras signaling, the tumorigenic properties of specific Ras-Raf complexes are not well characterized. Here, we examine the ability of individual Ras and Raf proteins to interact in live cells using bioluminescence resonance energy transfer (BRET) technology. We find that C-Raf binds all mutant Ras proteins with high affinity, whereas B-Raf exhibits a striking preference for mutant K-Ras. This selectivity is mediated by the acidic, N-terminal segment of B-Raf and requires the K-Ras polybasic region for high-affinity binding. In addition, we find that C-Raf is critical for mutant H-Ras-driven signaling and that events stabilizing B-Raf/C-Raf dimerization, such as Raf inhibitor treatment or certain B-Raf mutations, can allow mutant H-Ras to engage B-Raf with increased affinity to promote tumorigenesis, thus revealing a previously unappreciated role for C-Raf in potentiating B-Raf function. Copyright © 2019 Elsevier Inc. All rights reserved.

Citation

Elizabeth M Terrell, David E Durrant, Daniel A Ritt, Nancy E Sealover, Erin Sheffels, Russell Spencer-Smith, Dominic Esposito, Yong Zhou, John F Hancock, Robert L Kortum, Deborah K Morrison. Distinct Binding Preferences between Ras and Raf Family Members and the Impact on Oncogenic Ras Signaling. Molecular cell. 2019 Dec 19;76(6):872-884.e5