Catherine Ravel, Annie Faye, Sarah Ben-Sadoun, Marion Ranoux, Mireille Dardevet, Cécile Dupuits, Florence Exbrayat, Charles Poncet, Pierre Sourdille, Gérard Branlard
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 2020 MarA set of eight SNP markers was developed to facilitate the early selection of HMW-GS alleles in breeding programmes. In bread wheat (Triticum aestivum), the high molecular weight glutenin subunits (HMW-GSs) are the most important determinants of technological quality. Known to be very diverse, HMW-GSs are encoded by the tightly linked genes Glu-1-1 and Glu-1-2. Alleles that improve the quality of dough have been identified. Up to now, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of grain proteins is the most widely used for their identification. To facilitate the early selection of HMW-GS alleles in breeding programmes, we developed DNA-based molecular markers. For each accession of a core collection (n = 364 lines) representative of worldwide bread wheat diversity, HMW-GSs were characterized by both genotyping and SDS-PAGE. Based on electrophoresis, we observed at least 8, 22 and 9 different alleles at the Glu-A1, Glu-B1 and Glu-D1 loci, respectively, including new variants. We designed a set of 17 single-nucleotide polymorphism (SNP) markers that were representative of the most frequent SDS-PAGE alleles at each locus. At Glu-A1 and Glu-D1, two and three marker-based haplotypes, respectively, captured the diversity of the SDS-PAGE alleles rather well. Discrepancies were found mainly for the Glu-B1 locus. However, statistical tests revealed that two markers at each Glu-B1 gene and their corresponding haplotypes were more significantly associated with the rheological properties of the dough than were the relevant SDS-PAGE alleles. To conclude, this study demonstrates that the SNP markers developed provide additional information on HMW-GS diversity. Two markers at Glu-A1, four at Glu-B1 and two at Glu-D1 constitute a useful toolbox for breeding wheat to improve end-use value.
Catherine Ravel, Annie Faye, Sarah Ben-Sadoun, Marion Ranoux, Mireille Dardevet, Cécile Dupuits, Florence Exbrayat, Charles Poncet, Pierre Sourdille, Gérard Branlard. SNP markers for early identification of high molecular weight glutenin subunits (HMW-GSs) in bread wheat. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2020 Mar;133(3):751-770
PMID: 31907562
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