Evaluation of resazurin-based assay for rapid detection of polymyxin-resistant gram-negative bacteria.

Huaiyu Jia, Renchi Fang, Jie Lin, Xuebin Tian, Yajie Zhao, Lijiang Chen, Jianming Cao, Tieli Zhou

BMC microbiology 2020 Jan 08

filter terms:

Colistin resistance is considered a serious problem due to a lack of alternative antibiotics. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is a resazurin reduction-based technique that relies on the visual detection of bacterial growth in the presence of a defined concentration of colistin. The aim of this study was to evaluate the performance of the Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test in the detection of colistin susceptibility in common clinical Gram-negative bacteria. A total of 253 clinical isolates from a teaching hospital, including Acinetobacter baumanii (n = 58, 8 colistin-resistant), Pseudomonas aeruginosa (n = 61, 11 colistin-resistant), Klebsiella pneumoniae (n = 70, 20 colistin-resistant) and Escherichia coli (n = 64, 14 colistin-resistant) were tested in this study. The sensitivity and specificity of the Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test compared to Broth microdilution method was 100 and 99%, respectively. Our results suggest that Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test could be used as an accurate detection method for colistin resistance.

Citation

Huaiyu Jia, Renchi Fang, Jie Lin, Xuebin Tian, Yajie Zhao, Lijiang Chen, Jianming Cao, Tieli Zhou. Evaluation of resazurin-based assay for rapid detection of polymyxin-resistant gram-negative bacteria. BMC microbiology. 2020 Jan 08;20(1):7