The role of PDIA3 in myogenesis during muscle regeneration.

Chao Wang, Yuanjiao Zhu, Dan Wu, Zien Wang, Xiaoli Xu, Yan Shi, Gang Yang, Yongming Yu, Xi Peng

Experimental & molecular medicine 2020 Jan

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Beta 3 (β3) integrin plays an important role in the initiation of myogenesis in adult muscle. Protein disulfide isomerases (PDIs) can activate β3 integrin in various cells to promote cell migration, adhesion and fusion. However, the effect of PDIs on myogenesis during muscle regeneration has not been elucidated. Here, we report that PDIA3 expression is induced in regenerating myofibers. The inhibition of PDIA3 in muscle injuries in mice disrupts myoblast differentiation, impairs muscle regeneration, and ultimately aggravates muscle damage. Moreover, PDIA3 expression is upregulated and observed on the cell surfaces of myoblasts during differentiation and fusion. The inhibition of extracellular PDIA3 with an anti-PDIA3 monoclonal antibody attenuates β3 integrin/AKT/mTOR signal activity, inhibits myoblast differentiation, and blocks the fusion of myoblasts. Thus, PDIA3 may be a mediator of myoblast differentiation and fusion during muscle regeneration.

Citation

Chao Wang, Yuanjiao Zhu, Dan Wu, Zien Wang, Xiaoli Xu, Yan Shi, Gang Yang, Yongming Yu, Xi Peng. The role of PDIA3 in myogenesis during muscle regeneration. Experimental & molecular medicine. 2020 Jan;52(1):105-117