Exploiting the genome of Thinopyrum elongatum to expand the gene pool of hexaploid wheat.

One hundred and thirty four introgressions from Thinopyrum elongatum have been transferred into a wheat background and were characterised using 263 SNP markers. Species within the genus Thinopyrum have been shown to carry genetic variation for a very wide range of traits including biotic and abiotic stresses and quality. Research has shown that one of the species within this genus, Th. elongatum, has a close relationship with the genomes of wheat making it a highly suitable candidate to expand the gene pool of wheat. Homoeologous recombination, in the absence of the Ph1 gene, has been exploited to transfer an estimated 134 introgressions from Th. elongatum into a hexaploid wheat background. The introgressions were detected and characterised using 263 single nucleotide polymorphism markers from a 35 K Axiom® Wheat-Relative Genotyping Array, spread across seven linkage groups and validated using genomic in situ hybridisation. The genetic map had a total length of 187.8 cM and the average chromosome length was 26.8 cM. Comparative analyses of the genetic map of Th. elongatum and the physical map of hexaploid wheat confirmed previous work that indicated good synteny at the macro-level, although Th. elongatum does not contain the 4A/5A/7B translocation found in wheat.

Citation

Lauren Baker, Surbhi Grewal, Cai-Yun Yang, Stella Hubbart-Edwards, Duncan Scholefield, Stephen Ashling, Amanda J Burridge, Alexandra M Przewieslik-Allen, Paul A Wilkinson, Ian P King, Julie King. Exploiting the genome of Thinopyrum elongatum to expand the gene pool of hexaploid wheat. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2020 Jul;133(7):2213-2226

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PMID: 32313991

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