Yong Wang, Wenhua Su, Zihao Mai, Mengyan Du, Si Yu, Yangpei Liu, Xiaopin Wang, Tongshen Chen
Biochemical and biophysical research communications 2020 Jul 05We here used fluorescence imaging to explore the effect of co-overexpression of Mcl-1 and Bak/BH3-only proteins on mitochondrial morphology. The cells co-expressing CFP-Mcl-1 and YFP-Bak/BimL/Puma/tBid showed co-localization of Mcl-1 with Bak/Puma/BimL/tBid and also showed the inhibitory action of Mcl-1 on the Bak-, BimL-, Puma- or tBid-mediated cell death. Co-expression of Mcl-1 and Bak but not BH3-only proteins induced time-dependent mitochondrial swelling. Fluorescence resonance energy transfer (FRET) imaging proved the direct binding of Mcl-1 to Bak, BimL, Puma and tBid, respectively. In addition, Mcl-1 prevented Bak oligomerization by retrotranslocating Bak from mitochondria into cytoplasm. Moreover, Mcl-1-Bak complex exhibited a good co-localization with mitochondria, and co-expression of Mcl-1 and Bak for more than 24 h not only induced mitochondrial swelling but also impaired mitochondrial membrane potential. Collectively, co-expression of Mcl-1 and Bak but not BH3-only proteins significantly induced mitochondrial swelling and subsequent loss of mitochondrial membrane potential. Copyright © 2020 Elsevier Inc. All rights reserved.
Yong Wang, Wenhua Su, Zihao Mai, Mengyan Du, Si Yu, Yangpei Liu, Xiaopin Wang, Tongshen Chen. Co-expression of Mcl-1 and Bak induces mitochondrial swelling. Biochemical and biophysical research communications. 2020 Jul 05;527(4):866-873
PMID: 32430171
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