Signaling Through Purinergic Receptor P2Y2 Enhances Macrophage IL-1β Production.

The release of nucleotides during necrosis or apoptosis has been described to have both proinflammatory and anti-inflammatory effect on the surrounding cells. Here we describe how low concentrations of UTP and ATP applied during macrophage priming enhance IL-1β production when subsequently the NLRP3 inflammasome is activated in murine resident peritoneal macrophages. Deficiency or pharmacological inhibition of the purinergic receptor P2Y2 reverted the increase of IL-1β release induced by nucleotides. IL-1β increase was found dependent on the expression of Il1b gene and probably involving JNK activity. On the contrary, nucleotides decreased the production of a different proinflammatory cytokines such as TNF-α. These results suggest that nucleotides could shape the response of macrophages to obtain a unique proinflammatory signature that might be relevant in unrevealing specific inflammatory conditions.

Citation

Gonzalo de la Rosa, Ana I Gómez, María C Baños, Pablo Pelegrín. Signaling Through Purinergic Receptor P2Y2 Enhances Macrophage IL-1β Production. International journal of molecular sciences. 2020 Jun 30;21(13)

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PMID: 32630144

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