The structure and stability analysis of the pea seed legumin glycosylated by oligochitosan.

Rui Yang, Mengyao Liu, Yuqian Liu, De-Mei Meng, Tianhua Ma, Cheng-Tao Wang, Jie Liu, Qiaoe Wang, Zhongkai Zhou

Journal of the science of food and agriculture 2021 Feb

filter terms:

The functionality of pea proteins is relatively weak relative to that of soybean proteins, which limits the application of pea proteins in food and nutritional applications. Glycosylation is a promising approach to influence the protein structure and in turn change the functional properties of pea proteins. In this study, the effect of transglutaminase-induced oligochitosan glycosylation on the structural and functional properties of pea seed legumin was studied. Different oligochitosan-modified legumin complexes (OLCs) were prepared by applying different molar ratios of legumin to oligochitosan (1:1 to 1:4) induced by transglutaminase (10 U g-1 protein). Results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), glucosamine, and free amino analysis showed that the legumin could be covalently bonded with the oligochitosan and were influenced by the applying dose of the oligochitosan. Infrared spectroscopy, fluorescence, and scanning electron microscopy analysis indicated that the structure of the different OLC samples could be changed to different extents. Moreover, although the emulsifying activity decreased, the emulsification stability, thermal stability, and in vitro digestive stability of the OLCs were remarkably improved relative to that of the untreated legumin. Oligochitosan glycosylation could change the structure of the legumin and consequently improve its emulsification stability, thermal stability, and in vitro digestive stability. This study will facilitate the legumin functionalization by the glycosylation approach to fabricate protein-oligochitosan complex for potential food and nutritional applications. © 2020 Society of Chemical Industry. © 2020 Society of Chemical Industry.

Citation

Rui Yang, Mengyao Liu, Yuqian Liu, De-Mei Meng, Tianhua Ma, Cheng-Tao Wang, Jie Liu, Qiaoe Wang, Zhongkai Zhou. The structure and stability analysis of the pea seed legumin glycosylated by oligochitosan. Journal of the science of food and agriculture. 2021 Feb;101(3):1065-1075