Correlation Engine 2.0
Clear Search sequence regions

  • Age2 (2)
  • AP 3 (8)
  • Arf1 (2)
  • casein (2)
  • clathrin (2)
  • electron (1)
  • GAP (3)
  • gene (1)
  • golgi apparatus (1)
  • hops complex (1)
  • lipids (1)
  • mass (2)
  • small gtpase (1)
  • subunit (3)
  • vacuole (6)
  • vesicles coat (1)
  • vesicular transport proteins (2)
  • Vps41 (6)
  • YCK3 (1)
  • yeast (1)
  • Sizes of these terms reflect their relevance to your search.

    Heterotetrameric adapter (AP) complexes cooperate with the small GTPase Arf1 or lipids in cargo selection, vesicle formation, and budding at endomembranes in eukaryotic cells. While most AP complexes also require clathrin as the outer vesicle shell, formation of AP-3-coated vesicles involved in Golgi-to-vacuole transport in yeast has been postulated to depend on Vps41, a subunit of the vacuolar HOPS tethering complex. HOPS has also been identified as the tether of AP-3 vesicles on vacuoles. To unravel this conundrum of a dual Vps41 function, we anchored Vps41 stably to the mitochondrial outer membrane. By monitoring AP-3 recruitment, we now show that Vps41 can tether AP-3 vesicles to mitochondria, yet AP-3 vesicles can form in the absence of Vps41 or clathrin. By proximity labeling and mass spectrometry, we identify the Arf1 GTPase-activating protein (GAP) Age2 at the AP-3 coat and show that tethering, but not fusion at the vacuole can occur without complete uncoating. We conclude that AP-3 vesicles retain their coat after budding and that their complete uncoating occurs only after tethering at the vacuole. © 2020 The Authors. Published under the terms of the CC BY 4.0 license.


    Jannis Schoppe, Muriel Mari, Erdal Yavavli, Kathrin Auffarth, Margarita Cabrera, Stefan Walter, Florian Fröhlich, Christian Ungermann. AP-3 vesicle uncoating occurs after HOPS-dependent vacuole tethering. The EMBO journal. 2020 Oct 15;39(20):e105117

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 32840906

    View Full Text