Formation and Characterization of β-Lactoglobulin and Gum Arabic Complexes: the Role of pH.

Ziyuan Wang, Jie Liu, Jian Gao, Mengna Cao, Gerui Ren, Hunjun Xie, Mingfei Yao

Molecules (Basel, Switzerland) 2020 Aug 25

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Protein-polysaccharide complexes have received increasing attention as delivery systems to improve the stability and bioavailability of multiple bioactive compounds. However, deep and comprehensive understanding of the interactions between proteins and polysaccharides is still required for enhancing their loading efficiency and facilitating targeted delivery. In this study, we fabricated a type of protein-polysaccharide complexes using food-grade materials of β-lactoglobulin (β-Lg) and gum arabic (GA). The formation and characteristics of β-Lg-GA complexes were investigated by determining the influence of pH and other factors on their turbidity, zeta-potential, particle size and rheology. Results demonstrated that the β-Lg and GA suspension experienced four regimes including co-soluble polymers, soluble complexes, insoluble complexes and co-soluble polymers when the pH ranged from 1.2 to 7 and that β-Lg-GA complexes formed in large quantities at pH 4.2. An increased ratio of β-Lg in the mixtures was found to promote the formation of β-Lg and GA complexes, and the optimal β-Lg/GA ratio was found to be 2:1. The electrostatic interactions between the NH3+ group in β-Lg and the COO- group in GA were confirmed to be the main driving forces for the formation of β-Lg/GA complexes. The formed structure also resulted in enhanced thermal stability and viscosity. These findings provide critical implications for the application of β-lactoglobulin and gum arabic complexes in food research and industry.

Citation

Ziyuan Wang, Jie Liu, Jian Gao, Mengna Cao, Gerui Ren, Hunjun Xie, Mingfei Yao. Formation and Characterization of β-Lactoglobulin and Gum Arabic Complexes: the Role of pH. Molecules (Basel, Switzerland). 2020 Aug 25;25(17)