Synthesis and evaluation of a UMI-77-based fluorescent probe for selective detecting Mcl-1 protein and imaging in living cancer cells.

Jia Li, Xuben Hou, Jinzhuo Bai, Yi Zhou, Chen Chen, Xinying Yang, Hao Fang

Bioorganic & medicinal chemistry 2021 Jan 01

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Development of efficient fluorescent probes for detecting the overexpressed Mcl-1 protein in living cells is imperative for the diagnosis and treatment of cancers. In this paper, a new UMI-77 based fluorescent probe (DNSH), was synthesized and characterized. DNSH bound to the hydrophobic pockets of Mcl-1 protein tightly and the binding affinity was 20-fold higher than that of previous developed Mcl-1 probe. DNSH exhibited specific fluorescence response to Mcl-1 protein rather than other proteins. In the presence of Mcl-1 protein, fluorescence emission of DNSH can be switched on. Furthermore, fluorescence colocalization experiment demonstrated that DNSH can be successfully used for imaging mitochondrial Mcl-1 protein in human prostate cancer cells without a washing process. These results showed that DNSH may find useful applications in biological research such as tracking Mcl-1 protein in living biological specimens. Copyright © 2020. Published by Elsevier Ltd.

Citation

Jia Li, Xuben Hou, Jinzhuo Bai, Yi Zhou, Chen Chen, Xinying Yang, Hao Fang. Synthesis and evaluation of a UMI-77-based fluorescent probe for selective detecting Mcl-1 protein and imaging in living cancer cells. Bioorganic & medicinal chemistry. 2021 Jan 01;29:115850