The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis.

Plant science : an international journal of experimental plant biology 2021 Jan

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Mannan is a class of cell wall polysaccharides widespread in the plant kingdom. Mannan structure and properties vary according to species and organ. The cell walls of cereal grains have been extensively studied due to their role in cereal processing and to their beneficial effect on human health as dietary fiber. Recently, we showed that mannan in wheat (Triticum aestivum) grain endosperm has a linear structure of β-1,4-linked mannose residues. The aim of this work was to study the biosynthesis and function of wheat grain mannan. We showed that mannan is deposited in the endosperm early during grain development, and we identified candidate mannan biosynthetic genes expressed in the endosperm. The functional study in wheat was unsuccessful therefore our best candidate genes were expressed in heterologous systems. The endosperm-specificTaCslA12 gene expressed in Pichia pastoris and in an Arabidopsis thaliana mutant depleted in glucomannan led to the production of wheat-like linear mannan lacking glucose residues and with moderate acetylation. Therefore, this gene encodes a mannan synthase and is likely responsible for the synthesis of wheat endosperm mannan. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Citation

Yves Verhertbruggen, Axelle Bouder, Jacqueline Vigouroux, Camille Alvarado, Audrey Geairon, Fabienne Guillon, Mark D Wilkinson, Fabian Stritt, Markus Pauly, Mi Yeon Lee, Jenny C Mortimer, Henrik V Scheller, Rowan A C Mitchell, Cătălin Voiniciuc, Luc Saulnier, Anne-Laure Chateigner-Boutin. The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis. Plant science : an international journal of experimental plant biology. 2021 Jan;302:110693