Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis.

S O Abd Algaffar, A Verbon, W W J van de Sande, S A Khalid

Antimicrobial agents and chemotherapy 2021 Feb 17

filter terms:

We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC50, 0.016 µg/ml) followed by voriconazole (MIC50, 0.063 µg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective. Copyright © 2021 Abd Algaffar et al.

Citation

S O Abd Algaffar, A Verbon, W W J van de Sande, S A Khalid. Development and Validation of an In Vitro Resazurin-Based Susceptibility Assay against Madurella mycetomatis. Antimicrobial agents and chemotherapy. 2021 Feb 17;65(3)