miR-21 promotes expression of NF-κB and NLRP3 in mouse RAW264.7 cells by inhibiting A20].

Objective To investigate the effect of miR-21 on the expression of NF-κB and NLR family pyrin domain containing 3 (NLRP3) in RAW264.7 cells stimulated by lipopolysaccharide (LPS) and its possible mechanism. Methods Real-time fluorescent quantitative PCR was used to detect the expression of miR-21 after RAW264.7 cells were stimulated with 100 ng/mL LPS for 24 hours. miR-21 inhibitors or mimics were transfected to regulate the expression of miR-21 in RAW264.7 cells. After miR-21 inhibitors or mimics were stimulated by LPS, real-time quantitative PCR was used to detect its effect on the mRNA expression of interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and IL-1β, and Western blotting was used to detect the effects on the expression of NF-κB, NLRP3 and A20 proteins. Results The expression of miR-21 significantly increased when RAW264.7 cells were stimulated by LPS. The expression of IL-6, TNF-α, IL-1β, NF-κB and NLRP3 was raised when the expression of miR-21 was up-regulated. The expression of IL-6, TNF-α, IL-1β, NF-κB and NLRP3 was reduced when the expression of miR-21 was down-regulated. miR-21 targeted the inhibition of A20 expression. Conclusion miR-21 can promote the expression of NF-κB and NLRP3 in RAW264.7 cells and its mechanism may be related to the targeted inhibition of A20.

Citation

Binggang Zhao, Zhesheng Shi, Songtao Zhang, Chuanming Liu, Jiangang Xie, Wei Zhao. miR-21 promotes expression of NF-κB and NLRP3 in mouse RAW264.7 cells by inhibiting A20]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 2021 Feb;37(2):140-145

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PMID: 33504420

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