A resorufin-based red-emitting fluorescent probe with high selectivity for tracking endogenous peroxynitrite in living cells and inflammatory mice.

Huihui Su, Nannan Wang, Jiamin Wang, Han Wang, Jian Zhang, Weili Zhao

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2021 May 05

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Peroxynitrite (ONOO-) plays essential roles on various physiological and pathological processes of living systems as a short-lived and highly reactive nitrogen (RNS) specie. The construction of novel long-wavelength fluorescent probes with high specificity towards ONOO- for imaging in vivo is still demand urgently. About this work, a novel resorufin-based red-emitting fluorescent probe for tracking ONOO- has been constructed. The probe RFP exhibited high selectivity towards ONOO- anion over other analytes. Utilizing the probe, ONOO- could be directly observed by the naked eye. Furthermore, RFP was successfully applied for imaging endogenous ONOO- in RAW264.7 cells and inflammatory mice. This work offers a convenient method for monitoring the intercellur ONOO- that be expected to be applied for explaining the bio-functional roles of ONOO- in living system. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Huihui Su, Nannan Wang, Jiamin Wang, Han Wang, Jian Zhang, Weili Zhao. A resorufin-based red-emitting fluorescent probe with high selectivity for tracking endogenous peroxynitrite in living cells and inflammatory mice. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2021 May 05;252:119502