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A resazurin micro-assay was developed to quantify acidifying bacteria. The resorufin fluorescent signal was measured over time and the determined time to reach the max slope (TMS) was plotted against CFU (colony forming unit) counts. This dynamic assay enabled to quantify nine lactic acid bacteria and a Bacillus licheniformis strain despite the increasing acidity of the medium. Copyright © 2021 Elsevier B.V. All rights reserved.


Maritxu Labadie, Irina Randrianjatovo-Gbalou, Meriem Zaidi-Ait-Salem, Valérie Dossat-Létisse, Catherine Fontagné-Faucher, Claire-Emmanuelle Marcato-Romain. A dynamic resazurin microassay allowing accurate quantification of cells and suitable for acid-forming bacteria. Journal of microbiological methods. 2021 Apr;183:106172

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PMID: 33610595

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